Extremophyte CRISPR

CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) is a genetic editing scientific technique that can be used to increase, decrease, insert or remove genes from organisms. Exploring its application in plants could be helpful for understanding plants' stress responses in microgravity that could translate to improved agricultural practices on Earth, in space, or or other terrestrial bodies on exploration missions. In this TÜBİTAK UZAY-sponsored project "Extreme Salt Stress and CRISPR Gene Editing Efficiency in Plants Under Microgravity" (Extremophyte CRISPR), researchers will investigate the downregulation via the CRISPR technique of three genes involved in the stress response of Arabidopsis thaliana (thale cress, a member of the mustard family). The second aim will evaluate the salt stress tolerance of two plants - one salt-sensitive and one salt-tolerant - that will be germinated and grown in the International Space Station (ISS).  This work builds on previous microgravity investigations showing how microgravity affects the growth, movement and genetics of this plant, and could provide valuable insights into plant adaptation to extreme environments and help develop more resilient crops for agriculture.